Blu, Jewel, and the rest of their family, including Eduardo, Jewel's father, and Roberto, a childhood friend of Jewel's, are heading to Rio for a summer vacation, but things go wrong when a storm blows in, forcing the macaws to hide out in a hollow. From there, a being known as Glaux appears in the mist and sucks them away into the owl kingdoms. What strange enemies must be fought?

In the midst of a sudden storm, Blu and Jewel are torn from their home and dropped off on a distant island. As they try to make peace with the locals, they have a deeper mission in mind: fulfill the prophecy they were given and find their way back home...